Simultaneous Detection of Bluetongue Virus Serotypes Using xMAP Technology
نویسندگان
چکیده
منابع مشابه
xMAP Technology: Applications in Detection of Pathogens
xMAP technology is applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, enabling simultaneous detection and typing of pathogenic viruses, bacteria, parasites and fungi and also antigen or antibody interception. As an open architectu...
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To the Editor: In September 2008, the Israeli Veterinary Field Services were notifi ed of uncharacteristic disease on a dairy farm near the border with Lebanon in Rosh Ha Nikra, (online Appendix Figure, www.cdc.gov/ EID/content/16/12/2003-appF.htm). In November, blood samples were obtained from 5 cows, 4 of which were recovering from signs of infection with bluetongue virus (BTV). Virus isolati...
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The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...
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We studied the potential of red deer as bluetongue maintenance hosts and sentinels. Deer maintained detectable bluetongue virus (BTV) serotype 4 RNA for 1 year after the virus was cleared from livestock. However, the virus was not transmitted to yearlings. BTV serotype 1 RNA was detected in red deer immediately after its first detection in cattle.
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We utilized one-step multiplex reverse transcription-PCR (RT-PCR) and Luminex xMAP technology to develop a respiratory multiplex liquid-chip assay (rMLA) for simultaneous detection of 6 common respiratory viruses, including influenza virus type A (FluA) and type B (FluB), para-influenza virus type 3 (PIV-3), respiratory syncytial virus (RSV), human metapneumovirus (MPV) and a threatening virus ...
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ژورنال
عنوان ژورنال: Microorganisms
سال: 2020
ISSN: 2076-2607
DOI: 10.3390/microorganisms8101564